Bilirubin, Direct
Wako Product Name
Direct Bilirubin V
For In Vitro Diagnostic Use
Special Characteristics
- No interferences from lysed cells or turbidity due to chylomicrons or other serum components.
- High stability of reagents using vanadate as the chemical oxidizing reagent instead of older Bilirubin oxidase enzymatic or diazo coupling methods. Typical reagent stability of 1 year.
- Reagents are ready to use. Unlike older methods, no preparation is required.
Serum and plasma bilirubin measurement is widely used as a screening test for liver function. The methods most widely used for determination of bilirubin are the diazo coupling method and the bilirubin oxidase enzymatic method. However, these methods have disadvantages such as interference by coexisting substances and unsatisfactory stability of reagents after preparation. The Wako Direct Bilirubin V is based on a chemical oxidation method, utilizing vanadate as an oxidizing agent shows good correlation with conventional methods, practically no interference by coexisting substances, and is a convenient ready-to-use liquid type reagent.
| Intended Use | The Wako Direct Bilirubin V is an in vitro colorimetric assay for the quantitative determination of direct bilirubin in serum. |
|---|---|
| Associated Medical Conditions |
Liver Disease |
| Method | Vanadate Oxidation Reaction |
| Assay Linearity | Up to 20 mg/dL |
| Basic Procedure |
Sample: 10 µL R1: 280 µL R2: 70 µL Wavelength: main: 450 nm, sub: 546 nm |
Ordering Information
| Catalog No. | Product Name | Pkg Size | Storage |
|---|---|---|---|
| 992-23691 | Direct Bilirubin V Buffer | 4 x 70 mL | 2-10°C |
| 994-23891 | Direct Bilirubin V Vanadate | 4 x 18 mL | 2-35°C |
| 996-23591 | Direct Bilirubin V Buffer | 4 x 540 mL | 2-10°C |
| 998-23791 | Direct Bilirubin V Vanadate | 4 x 135 mL | 2-35°C |
Calibrator
| 993-73291 | Bilirubin Calibrator |
